Amyris Brasil, a subsidiary of 4229  Amyris, has announced that it will  supply renewable diesel during a 12-month fleet test involving 20 city  buses in Rio de Janeiro, Brazil. The renewable fuel derived from  sugarcane, known locally as Diesel de Cana, will be blended at a 30 per  cent rate with petroleum-derived diesel and used in 1685  Mercedes-Benz buses  operated by Viação Saens Peña, a Rio-based bus operator. The Rio  transportation federation, Fetranspor, will use the data collected  during this fleet test to evaluate the engine and environmental benefits  of Amyris’s renewable diesel. The results of the fleet test will be  presented at the United Nations Conference on Sustainable Development  (Rio+20) taking place in Rio in June 2012.
 The city of Rio de  Janeiro has more than 8,000 buses consuming about 280 million litres of  diesel per year. The Rio fleet test is expected to validate the  significant reduction of nitrogen oxide and particulate matter emissions  evidenced in recent Mercedes-Benz engine tests with a 30 per cent blend  of Amyris’s renewable diesel.